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CLS Cell Lines Service GmbH
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China Center for Type Culture Collection
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Shanghai Genechem Ltd
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Dainippon Sumitomo
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Gemini Bio
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Pasteur Institute
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Image Search Results
Journal: Cancers
Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors
doi: 10.3390/cancers17213517
Figure Lengend Snippet: NIPP-induced alterations in extracellular H 2 O 2 levels, intracellular ROS production, and MMP in tumor cells. ( A ): H 2 O 2 level in the cell culture medium RPMI 1640 and DMEM/F12, and PBS after treatment with NIPP ( a ); NO 2 − level in the cell culture medium RPMI 1640 and DMEM/F12, and PBS after treatment with NIPP ( b ); NO 3 − level in the cell culture medium RPMI 1640 and DMEM/F12, and PBS after treatment with NIPP ( c ). ( B ): ROS level inside the tumor cells SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), MDA-MB-231 ( f ) cells, after treatment with the NIPP in 30 min ( n = 6). All data are normalized to the control group; ( C ): Mitochondria membrane potential relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), MDA-MB-231 ( f ) cells after the exposure of NIPP 24 h, 48 h, and 72 h. Every experiment was replicated at least 3 times. All data are normalized to the control group. *** p < 0.001, **: p < 0.01, *: p < 0.05.
Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany),
Techniques: Cell Culture, Control, Membrane
Journal: Cancers
Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors
doi: 10.3390/cancers17213517
Figure Lengend Snippet: NIPP inhibits the proliferation of tumor cells. ( A ): Cell growth curves for SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells. The groups were categorized based on their distance from NIPP to the medium surface: the control group, 1 cm group, 3 cm group, and 6 cm group. All groups, except the control group, were exposed to NIPP for 4 min. Each experiment was conducted at least three times. ( B ): Visualization of NIPP treatment tumor cells with different distances. ( C ): Cell growth curves for SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells. The groups were organized based on the treatment time of NIPP for the tumor cells: the control group, 1 min group, 2 min group, and 4 min group. Except for the control group, the distance of NIPP from the culture medium was 1 cm for all groups. ( D ): Visualization of NIPP treatment tumor cells with different duration. Each experiment was repeated at least three times. ( E ): Wound-healing Test of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells at 0 and 24 h. Each experimental group was compared with the control group.
Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany),
Techniques: Control
Journal: Cancers
Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors
doi: 10.3390/cancers17213517
Figure Lengend Snippet: NIPP treatment alters the cytoskeletal organization of tumor cells and glucose consumption in tumor cell culture medium. ( A ): Immunofluorescence of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells. Green represents F-actin, and Blue represents the Nucleus. Magnification 20×. Next row of pictures are partial screenshots from the previous row. ( B ): Glucose relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( C ): Lactate relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 24 h, 48 h, and 72 h. Every experiment was replicated at least 3 times. Each experimental group was compared with the control group. ***: p < 0.001, **: p < 0.01, *: p < 0.05.
Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany),
Techniques: Cell Culture, Immunofluorescence, Control
Journal: Cancers
Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors
doi: 10.3390/cancers17213517
Figure Lengend Snippet: NIPP increases LDH release in tumor cell cultures and induced no significante alterations of SOD levels in tumor cell. ( A ): LDH relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 30 min and 24 h. ( B ): SOD relative level in the medium of SKOV-3 ( a ), OVCAR-3 ( b ), LNCaP ( c ), PC-3 ( d ), MCF-7 ( e ), and MDA-MB-231 ( f ) cells after the exposure to NIPP for 24 h. Every experiment was replicated at least 3 times. Each experimental group was compared with the control group. ***: p < 0.001, **: p < 0.01, *: p < 0.05.
Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany),
Techniques: Control
Journal: Cancers
Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors
doi: 10.3390/cancers17213517
Figure Lengend Snippet: ( A ): Quantification of HSP27 relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7, and MDA-MB-231 cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. The uncropped bolts are shown in .
Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany),
Techniques: Expressing, Western Blot, Control
Journal: Cancers
Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors
doi: 10.3390/cancers17213517
Figure Lengend Snippet: ( A ): Quantification of HSP40 relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7, and MDA-MB-231 cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .
Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany),
Techniques: Expressing, Western Blot, Control
Journal: Cancers
Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors
doi: 10.3390/cancers17213517
Figure Lengend Snippet: ( A ): Quantification of HSP70 relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7, and MDA-MB-231 cells after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .
Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany),
Techniques: Expressing, Western Blot, Control
Journal: Cancers
Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors
doi: 10.3390/cancers17213517
Figure Lengend Snippet: ( A ): Quantification of HSP90α relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7 cells, and MDA-MB-231 after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. They were statistically evaluated with Student t -test. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .
Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany),
Techniques: Expressing, Western Blot, Control
Journal: Cancers
Article Title: Non-Invasive Physical Plasma as an Oncological Therapy Option: Modulation of Cancer Cell Growth, Motility, and Metabolism Without Induction of Cancer Resistance Factors
doi: 10.3390/cancers17213517
Figure Lengend Snippet: ( A ): Quantification of HSP90β relative expression level in SKOV-3, OVCAR-3, LNCaP, PC-3, MCF-7 cells, and MDA-MB-231 after the exposure to NIPP for 24 h, 48 h, and 72 h. ( B ): Western blot signals. Each experiment was replicated at least 3 times. Experimental groups were compared to control group. They were statistically evaluated with Student t -test. **: p < 0.01, *: p < 0.05. The uncropped bolts are shown in .
Article Snippet: SKOV-3 (Cryovial: 300343, Cell Lines Service GmbH, Heidelberg, Germany), OVCAR-3 (Cryovial: 300307, Cell Lines Service GmbH, Heidelberg, Germany),
Techniques: Expressing, Western Blot, Control
Journal: British Journal of Cancer
Article Title: Insulin-like growth factor binding protein-6 inhibits prostate cancer cell proliferation: implication for anticancer effect of diethylstilbestrol in hormone refractory prostate cancer
doi: 10.1038/sj.bjc.6602520
Figure Lengend Snippet: Inhibition of the proliferation of human prostate cancer cell lines LNCaP and PC-3 by diethylstilbestrol (DES). Cells were incubated for 24 h, and thereafter CM was aspirated away and the cells were incubated with CM containing various concentrations of DES. After 72 h, the number of viable cells was measured by MTT assay. Optical densities (OD) of cell lysates were measured at a wavelength of 540 nm. The values are expressed as means+s.d. ( n =3), and the P -values were <0.05 ( * ) and <0.01 (†).
Article Snippet: The
Techniques: Inhibition, Incubation, MTT Assay